Journal: Frontiers in endocrinology
Article Title: Type 1 diabetes impairs the activity of rat testicular somatic and germ cells through NRF2/NLRP3 pathway-mediated oxidative stress.
doi: 10.3389/fendo.2024.1399256
Figure Lengend Snippet: FIGURE 3 Analysis of LC activity in control and T1D rat testis. (A) Testicular Testosterone levels and (B) WB analysis of StAR, 3b-HSD, CYP17A1, and CYP19A1 protein levels. (C–F) Histograms showing StAR, 3b-HSD, CYP17A1, and CYP19A1 relative protein levels quantified using ImageJ and normalized to b- actin. (G) Testicular StAR (red) and 3b-HSD (green) immunolocalization. The slides were counterstained with DAPI-fluorescent nuclear staining (blue). The images were captured at ×20 (scale bars = 20 µm) magnification and ×40 (scale bars = 10 µm) for the insets. Asterisks, LC. (H, I) Histogram showing the quantification of StAR and 3b-HSD fluorescence signal intensity, respectively. (J) WB analysis of INSL3 and RXFP2 protein levels. (K, L) Histograms showing INSL3 and RXFP2 relative protein levels quantified using ImageJ and normalized to b-actin. All values are expressed as means ± SEM from five animals in each group. *p < 0.05; **p < 0.01.
Article Snippet: Testicular testosterone level measurement Testosterone (T) levels were determined in the testis of both groups using a commercial kit (#582701; Cayman Chemical Company, Michigan, MI, USA) and using a previously published protocol for the sample’s treatment (45).
Techniques: Activity Assay, Control, Staining